检测葡萄酒中SO2的含量

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Analysis And Testing TechnologyApplication JulaboVISCOAnalysis And Testing TechnologyApplication USE This application note descripe the titration procedure with iodine solution. APPLIANCES Titrator： TL 7000 or TL 7750 M1 Basic device Magnetic stirrer TM 235 20 mL Exchange unit WA 20， with amber glass bottle for the titrant， complete Option： autosampler TW alpha plus 24 or TW 7400+2x TITRONIC universal ELECTRODES Electrode： Pt 1200 with L1NN or Pt 1400 with autosamplerElectrode cable： L 1 NN REAGENTS Solvent： water dest. Standardisation： Na2S203Titrant： lodine sol. (12)0.025 m or 0.01 m other reagents H2SO4 10%， NaOH 4 mol/l， KI-solution 5% and EDTA-Na2 DESCRIPTION Preparation of lodine Solution We recommend ready to use agents. The lodine solutions are also available in ampoules. Application A) Determination of the free SO2 Pipette 50 ml of the sample in a 100 or 150 ml glass beaker (room temperature->20 °C)， add 3 ml H2SO4 10 % +30mg EDTA-Na2， 10 ml Kl solution and titrate immediately with the iodine solution. B) Determination of total SO2 Add 8 ml 4 m NaOH to the titrated sample A and wait 5 minutes. Add then 10 ml H2SO4 while stirring and titrateimmediately with the iodine solution. Better results could be reached if 20 ml NaOH are added again to the titrated sample and a reaction time of 5minutes is given. After the addition of H2SO4 the titration is started again. Take the summary of both titrations of thetotal SO2 titration to calculate the result. Electrode handling The dead stop electrode can be stored dry after use. LITERATURE Amtsblatt der Europaischen Gemeinschaft. Dr. Alfred Schmitt Aktuelle Weinanalytik Verlag Heller Chemie- und Verwaltungsvorschriften mbH； Titration Method： SO2 free， page 1 GLP documentation Titration graph 2.0pAL1413ml ·ml · pA/ml Method data Method name： SO2 in wine Titration duration： 9s End date： 22.02.13 End time： 10：24：18 Titration data Sample ID： White wine Pattem： 25.000 ml Start pA： 0.192 uA End uA： 2.793 pA EP： 1.413 ml/ 2.0 pA SO2： 1.8mg/l Calculation formula S02： (EP-B)*T*M*F1/(V*F2) Mol (M)： 32.00000 Blank value (B)： 0.0000 ml Titre (T)： Factor 1 (F1)： 1.0000 Pattem (V)： 25.000 ml (m) Factor 2(F2)： 1.0000 Statistics： Off Factor 2(F2)： 1.0000 Statistics： Application Titration Method： SO2 free， page 1 Method data overall view Method name：502 in wineCreated at：02/22/1310：18：41Method type：Automatic titrationLast modification：02/22/1310：20：53Measured value：uATitration mode：d-stopDocumentation：GLPLinear steps：0.040 mlMeasuring speed / drift：1sInitial waiting time：0 sTitration direction：IncreasePretitration：OffEndpoint：2.0uAdelta endpoint：1.0 uAEndpoint delay：5sPolarization voltage：100 mVDosing parameterDosing speed：60.00 %Filling speed：30 sMaximum dosing volume：20.00 mlUnit valuesUnit size：50mlUnit ID：10045002Reagent：Iodid/IodatBatch ID：no entryConcentration [mol/l]：1.00000Determined at：08/31/1223：19：38Expire date：09/29/12Opened/compounded：08/29/12Test according ISO 8655：06/01/12 Last modification： 11/22/12 15：47：54 Application NOTES If you are using an autosampler then the addition of H2SO4 and KI have to be done with a piston burette such asTITRONIC universal direct before the titration of the SO. lf you should have any questions concerning application， please contact the Application Department of SI Analytics；tel.： +496131665062 or 5118 JULABO TECHNOLOGY(Beijing)Co.，LTD地址：北京市朝阳区酒仙桥东路1号院M8号楼C厅3层301室 Add ： Room 301，C hall，M8 office building，No.1 jiuxianqiao east road ，Chaoyang District，Beijing，ChinamTel.： 4008092068 昌Fax ： +86 (0)10 64390585E-Mail ： info@julabo.cn优莱博技术(北京)有限公司 page from date： Web： www.julabo.cn，www.julabo-visco.com