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Automated protocols to facilitate sample preparation in lipidomics have been previously developed. The aim of these procedures is to minimize human errors resulting from handling large quantities of samples, and to decrease the amount of time spent in sample preparation. This application note describes the semi-automated extraction of phospholipids and sphingolipids from human plasma using the Agilent Bravo Automated Liquid Handling Platform. This procedure includes the transfer of the starting material (plasma is usually delivered in 1.5-mL tubes) to 96-well plates, which can be either stored or used for the lipid extraction. In the latter case, a butanol/methanol (BuMe) mixture is added to the sample. Although the samples are still transferred manually to the sonicator and the centrifuge during the lipid extraction procedure, the reported protocol allows a high number of samples to be processed uniformly, decreasing the sample preparation time and yielding high reproducibility according to the results obtained after analysis of the extracted lipids with an Agilent UHPLC/MS/MS system.

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A Semi-Automated LipidExtractionProtocol Using theAgilent BravoAutomated Liquid Handling Platform Abstract： Automated protocols to facilitatesample pireparationnlinlipidomicshavebeenpreviously developed. The aim of these procedures is to minimize human errors resultingfrom handling large quantities of samples， and to decrease the amount of time spentin sample preparation. This application note describes the semi-automated extractionof phospholipids and sphingolipids from human plasma using the Agilent Bravo AutomatedLiquid Handling Platform.This procedure includesthe transfer of the startingmaterial (plasma is usually delivered in 1.5-mL tubes) to 96-well plates，，which canbe either storedOr used for： thelipidextraction.1.Inthe lattercase， abutanol/methanol (BuMe) mixture is added to the sample. Although the samples are stilltransferred manually to the sonicator and the centrifuge during the lipid extractionprocedure， the reported protocol allows a high number of samples to be processeduniformly， decreasing the sample preparation time and yielding high reproducibilityaccording to the results obtained after analysis of the extracted lipids with anAgilent UHPLC/MS/MS system. Agilent Technologies Automated protocols to facilitate sample preparation in lipidomics have been previously developed. The aim of these procedures is to minimize human errors resulting from handling large quantities of samples, and to decrease the amount of time spent in sample preparation. This application note describes the semi-automated extraction of phospholipids and sphingolipids from human plasma using the Agilent Bravo Automated Liquid Handling Platform. This procedure includes the transfer of the starting material (plasma is usually delivered in 1.5-mL tubes) to 96-well plates, which can be either stored or used for the lipid extraction. In the latter case, a butanol/methanol (BuMe) mixture is added to the sample. Although the samples are still transferred manually to the sonicator and the centrifuge during the lipid extraction procedure, the reported protocol allows a high number of samples to be processed uniformly, decreasing the sample preparation time and yielding high reproducibility according to the results obtained after analysis of the extracted lipids with an Agilent UHPLC/MS/MS system.

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