水中布洛芬检测方案(液质联用仪)

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52854PKI HPLC Analysis ofIbuprofen in Water Usinga Quasar SPP C18 Column unused drugs via drains and toilets.1 A report found that the six most consistenthighest reported concentrations in finished drinking water for active pharmaceuticalingredients (APIs) were from ibuprofen， triclosan， carbamazepine， phenazone，clofibric acid， and acetaminophen.’ Ibuprofen (Figure 1) is a widely used analgesic and antipyretic for adults and childrenand since it's one of the most reported pharmaceutical in finished drinking waste，it is important to determine the amount removed by different water treatmentprocesses.2.3 HPLC is an essential technique to determine the quantity of ibuprofen inwater and help optimise a process for its removal. This application brief describes theuse of a Quasar superficially porous particles (SPP) C18 column for the analysis ofibuprofen in water . Introduction Pharmaceuticals are continuouslventering our waterways via humanexcretion of incompletely absorbedmedication and improper disposal of Figure1. Chemical structure ofibuprofen. Experimental Conditions Method Parameters Table 1. HPLC method parameters. Instrument PerkinElmer FlexarM with PDA PlusM Detector Column Quasar SPP C18 150x4.6 mm， 2.6 um P/N： N9308910 Mobile Phase 60：40， ACN ： H，0 (0.1 % phosphoric acid) Flow Rate 0.6 ml/min Temperature 25°C Wavelength 220 nm Injection Volume 3 pL Analyte Ibuprofen Solvents and Reagents All solvents and reagents used were HPLC grade. A stocksolution of ibuprofen (1 mg/mL) was prepared in acetonitrile.From this solution， a working standard solution was prepared(0.2 mg/mL) by dilution with distilled water. Before injection intothe HPLC system， the standard was filtered using a 0.45 pmPTFE syringe filter (P/N： 02542885). Results Superficially porous particles， SPP， (also called： shell， fused-core，core-shell， partially porous， pellicular) are made of a solid， non-porous core surrounded by a shell of a porous silica material thathas properties similar with those of the fully porous silica materialsconventionally used in HPLC. The terminology of "fused-core"was introduced by Jack Kirkland. As the name implies， fused-coreparticles are manufactured by "fusing" a porous silica layer onto asolid silica particle (Figure 2). Such phases can be used onstandard HPLC instrumentation， without worrying about highbackpressure or compromising column longevity. With a shorterdiffusion path with the SPP particle itself， coupled with a uniformpacked bed and ultra-inert silica surface， reductions in run timescan be observed. Use of these SPP columns also often results inhigher kinetic efficiencies and savings in solvent consumption. The analysis of ibuprofen in water using a Quasar SPP C18(150x4.6 mm， 2.6 pm) column is shown in Figure 3. Ideallysuited to the analysis of small molecules， the Quasar SPP C18phase provides high efficiency for the analysis of ibuprofen inwater (17，011 N using the Foley-Dorsey approximation method).Good peak shape is also observed due to the ultra-high puritysilica base and low residual silanol activity. The run time of themethod could be reduced by increasing the flow rate. Figure 2. Comparison of a conventional fully porous particle (left) and a superficiallyporous particle (right). Figure 3. HPLC analysis ofibuprofen. Conclusion Good retention and peak shape for the analysis of ibuprofen inwater is observed on the Quasar SPP C18 phase. RRun time could be reduced by using a faster flow rate.S SPP columns provide faster run times in comparison withconventional fully porous particle columns， as well as higherkinetic efficiencies and savings in solvent consumption. The ultra-high purity silica base and low residual silanol activityyields excellent peak shape. References 1. J. Bound and N. Voulvoulis， Predicted and measuredconcentrations for selected pharmaceuticals in UK rivers：implications for risk assessment， Water Research， 40，2006，2885-2892. 2. C.G. Daughton， Pharmaceutical Ingredients in Drinking Water：Overview of Occurrence and Significance of Human Exposure.Chapter 2， R. Halden (ed.)， Contaminants of Emerging Concernin the Environment： Ecological and Human HealthConsiderations. American Chemical Society， Washington， DC，1048：9-68， (2010). 3. R. Bushra and N. Aslam， An Overview of Clinical Pharmacologyof Ibuprofen， Oman Medical Journal， 25，2010， 155-1661. Consumables Used Component Description Part Number Column Quasar SPP C18 (150x4.6 mm， 2.6 pm) N9308910 HPLC Vials 2 mL， 9 mm Screw Top Vial with Write-on Patch and Fill Lines (100/pack) N9307802 HPLC Vial Caps 9 mm Screw Top Blue (polypropylene) Cap with PTFE/Silicone pre-slit Septa (100/pack) N9306203 Syringes Syringe 1 mL BD Luer-Lok Disposable， Pack of 100 02542890 Syringe Filters 0.45 um PTFE syringe filter 02542885 PEEK Fittings Fingertight for 1/16"OD PEEK tubing 09920513 PerkinElmer， Inc.940 Winter StreetWaltham， MA 02451 USAP： (800) 762-4000 or(+1) 203-925-4602 www.perkinelmer.com For a complete listing of our global offices， visit www.perkinelmer.com/ContactUs ( C opyright ◎2020， PerkinElmer， Inc. All rights r e s e r ved. Pe r kin E lmer@ is a r egi s tered trade m ark of PerkinE l mer， Inc. A l l other t ra d emar k s ar e the proper t y of their res p ectiv e owners . ) 布洛芬是一种广泛应用于成人和儿童的止痛和解热剂，由于它是末梢水中报道最多的药物成份之一，因此确定不同水处理工艺的去除量很重要。高效液相色谱法是确定布洛芬在水中的含量和帮助优化其去除工艺的关键技术。本文简要介绍了Quasar表面多孔颗粒(SPP) C18色谱柱在水中布洛芬分析中的应用。