CPL-300圆偏振发光光谱仪测定樟脑醌的CPL

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通过将CPL与ECD相结合，可以获得更多关于手性分子的结构信息。为了以高分辨率测量尖锐的CPL峰值，CPL光谱仪使用两个棱镜单色仪。发射和激发单色仪都配备了连续可变的狭缝驱动器，可以选择适当的波长和带宽。Application NoteCD-0031 CPL Measurement of Camphorquinone Using CPL-3002/3Application NoteCircularly Polarized Luminescence Spectrometer CPL Measurement of Camphorquinone Using CPL-300Circularly Polarized Luminescence Spectromete r When c hir al com p ound s are excited wit h un p olar iz ed l i ght the dif f e renc e i n em i ssion i n t e n s i t ie s of lef t- a n d r ight -ha n ded circularly polar iz e d l ight can be meas u red. T h is phe n ome n on is called circularly polar i zed l uminescence (CPL). While ci r cular dich r oism provides informa t i on about the ground state of chiral molecules， CPL spectroscopy probes t h e excited states of ch ir al molecules . CPL-300 C ir cularly Po l arized Lumi n escence Spectro p hotomete r By combining CPL wit h ECD， mor e st ru ctural in formation r e gardi n g chiral mol e cul e s ca n b e obta in ed. To m easur e s h arp CPL p e aks at a high resolut i on， the CPL spect r ometer uses two p r i sm monoc h romators. Bot h the emission and excitation monoc h romators are equipped with cont i nuously variable slit drives， w h ich allow f or an appropr i a t e wavelengt h and band width s e l e ct i on T h is application n ote demonstrates t he measurement a n d analysis of camphorquinone u sing two complime n tary t e c h niqu e s ： c ir cular dichroism and c i rcularly pola rize d lumin e sc e nce. Keywords C ir cu l ar l y p o la r ized lu m in es c en c e， C ir cu l a r d ichr o i sm， CD-0031， M aterials 28600 Ma r y 's C ou r t， Easton， M D 21601 US A Experimen t al 18 mM of (1R)-(-)-and (1S )-(+)-ca mph o r q u i n on e w as prepared in e th a n o l . CD CPL Data Pitch 0.1nm Excitation Wavelength 440 nm Scan Speed 50 nm/min Excitation Bandwidth 16nm D.I.T. 2 sec Data Pitch 0.1nm Bandwidth 1nm Emission Bandwidth 10 mm Accumulations 1 Scan Speed 50 nm/min D.I.T. 4 sec Accumulations 16 Results The CPL and f luorescence spectra of (1R)-(-)- and (1S)-(+)-camphorquinone were measured using a CPL-300， while CD and absorpt i on were collected using a J-1500. The CD and CPL sig n als were norma l ized as ga bs =A e/e a n d gj m =AI/l，r espect i ve l y. T h e spect r a are shown i n Figu r e 1. Figure 1. Th e (a ) CD and C P L s pec t ra ， (b) a b sor ba n c e and f luor es c en c e sp e c t ra ， a n d (c)g and gspe c tra o f t h e (1R)-(-)-(bluere d ) an d (1S )-(+)-(bl u er ed) camph or q u in one et h anol s ol u t ion . 28600 Mary's Cour t ， E a s to n ， M D 21601US A Appl i c a t i on Note C onclusion The results are in good agreement with t h e camphorquinone CPL spectrum previously reported. 1. Chun Ka L u k and F . S . Ric h ardson ， JACS. 1974， 96，2006-2009 28600 Mary's Cour t ， E a s to n ， M D 21601US A