使用IMV-4000分析外来化合物

智能文字提取功能测试中

IMV-4000是具有16元件线性阵列MCT检测器的FTIR显微镜。IMV-4000线性阵列MCT探测器与高速扫描FTIR、精密自动载物台和并行数据处理相结合，可实现每分钟9600点的测绘测量。测量可以比使用单个元件检测器进行的传统映射测量快100倍。当使用单个元件检测器来测量其中存在多个成分的样本时，可能难以识别观察到的图像中的单个成分，因为这些成分可能是不均匀分布的，并且可能同时测量多个成分。在光谱是几个成分的集合的情况下，当使用库搜索程序进行定性分析时，可能很难准确地分离和分析每个成分，因为光谱库的数据库通常由单个成分组成。使用具有窄孔径和长积累的单元件探测器可以进行映射测量并分离多种异物，但识别所有成分可能需要非常长的时间。线性阵列FTIR显微镜可以快速进行广域映射测量，用于生物样品、多层膜和其他类型样品的表征，并且可以通过使用非常小的测量孔径有效地分离空间分布紧密的多个组分。在本申请说明中，使用IMV-4000对污染树脂样品的异物进行IR图谱绘制。在进行映射测量后，使用库搜索程序来分析在映射中获得的特征光谱。鉴定出三种成分：纤维素、聚酯和有机硅。JASCO的映射分析程序用于基于光谱中峰值的高度或面积创建测量区域的图像映射，并同时在RGB显示器中可视化多个图像映射；该函数用于通过覆盖每个键带的3D彩色视图来确认三个分量的分布。Application Note030MT0194-E Analysis of Foreign Compounds2/3Application Noteusing IMV-4000 The I MV-4000 is an FT I R microscope wit h a 16-elemen t l i nea r array MCT detector. The I MV-4000 linear array MCT detector combined with a high-speed scanning FTIR， precision auto-stage， and parallel data process i ng enables mapping measurement of u p to 9，600points pe r minute. Measurement can be made up to 100 times faster than conventional mapping measurements made using a single element detector. FT/IR-4000 and 6000 Series Spectrometers When using a single-eleme n t detector to measu r e sa m ples i n wh i c h there a r e mu l t i ple components， i t may be difficult to identify a single compone n t in an obse r ved i mage because the compone n ts may be no n -uniform l y distributed and it is likely that multiple components wi ll be measured simu lt a n eously. In the case where a spectrum i s a n aggregate of several components ， and when qual i tative analysis i s made using a l i brary sea r ch program it ca n be d i f f icult to accurately separa t e and analyze each component because t h e database of spectral l i brar i es are usual l y comprised of single components. I t is possible to perform mapping measurements and separate mult i ple f oreign substances using a single-element detector with a nar r ow apertu r e and l ong accumula t i ons， bu t it can take an extreme l y long time to identify all the components . A li n ea r a r ray FT I R microscope can quickly perform wide area mapping measu r ements for the characterization of biological samples， multilaye r films and others types of samples， and it can ef fi c i en t ly separate mult i ple components tha t a r e closely spatially distributed by using a very small measu r ement ape r ture. I n this application note， an I MV-4000was used to perform IR mapping of foreign substances contaminating a resi n sample. After mapping measurement wa s performed， a library search prog r am was used to analyze the c h aracteristic spectra obtained in the map. Three components were i dentified： cellulose， polyester， and organic silicon .JASCO's mapping analysis program was used to c r eate image maps of the measured area based on the height or area of the peaks i n the spectra ， as well as simultaneously visua l izing multiple image maps in an RGB display； t h is fu n ction was used to confirm the distribution of the th r ee components by over l aying a 3D-colored view of each key band. Keyword s MCT detector， l in ear array， multi p le components and IMV-4000 Exper i men t al T h e f oreign substa n ces co n tain e d w i t h i n the resin were ext r act e d ， a plat e was made u sin g KBr， and mic r osco p ic measur e me n t was p e rformed usi n g t r ansm i ssion mea s urement. T he for e ig n su b stanc e s wer e meas ure d and id entifi e d bas e d on t h e obtai ne d sp e ct r a . Figure 1. Mid-I R sp e c t ra of c i s-f at s and tran s-f at s (d e mon s t r atin g t h e si g n i fic a n t pe a ks i n th e highl i g h t e d a rea s ) Condition Detector Linear array MCT (16 elements) Resolution 8cm² Accumulation 50 Measurement Method Transmission Objective Mirror 16x cassegrain Measurement Time Approx. 10 minutes Measurement Area 600x600 um (48x48 points) Measurem e n t Resul t s Spectra were measured of t h ree diffe r ent foreign compounds found in t he sample and a Biorad KnowltAll @ search was made. The library sea r ch conf i rmed the i dent i t i es of eac h of the foreign com p o u nd s contained in the sample. JASCO USA 28600 Mary's Cour t ， E a s to n ， M D 21601US A UASGO T el ： (800) 333-5272， Fa x ： (410) 822-7526 A ppl ic a tio n Lib r ary： ja s c o i n c.com /appli c a t i o ns -Result of Sadtl e r s pectra search Spectra of foreign compounds Figure 2. Se a rc h re s ult 1 (C el l ulos e) Figure 3. Sea r ch r esu lt 2 (P o ly e ster ) Figure 4. Sea r ch r esult 3 (Organi c s i lico n ) T h e p e aks sp e c i fic to e ach com p o n e n t in the a b ove sp e c t ra are c l assifi e d by co l or. Figu re 5 i s a distributio n map of t h e s p ec i f i c p eaks and an obs e rv e d i mag e of f or e ign com p ounds. X [um] Figure 5. Ob s er v e d i m a ge o f fore ign co mp oun ds and d i st ri bu tio n (RGB d i sp l a y)Gree n ： C el l ulose (3344 c m) R e d ： Pol y es t e r (1261 c m ) B lue： O rgani c silicon (1800 c m ) 28600 Mary's Cour t ， E a s to n ， M D 21601US A USGO