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yubo726
第26楼2010/04/14
Nonlinear Through Zero
If you expect the concentrations of your samples to be outside the linear range of the calibration curve , select this option. This option covers the widest concentration range. This calibration curve is forced to go through the point defined by the calibration blank which is set at zero absorbance and zero concentration. The equation for Nonlinear zero intercept is:
C=K0*(K1*A+K3*A*A)/(K2*A-1)
In this expression, C is the concentration, A is the observed absorbance or emission, K1, K2, and K3 are coefficients determined during the calibration procedure, and K0 is the reslope coefficient that is set equal to 1.0 during the initial calibration. When the number of calibration standards used exceeds the number of coefficients, the method of least squares is used to determine the coefficients.
The blank is always analyzed first, and the reading obtained is automatically subtracted from the readings from all subsequent calibration standards.
Linear, Calculated Intercept
If you expect the concentrations of your samples to be within the linear range of the calibration curve, select this option. This is often the case at low absorbance values and low concentration values. The calibration blank is treated as another calibration standard by the system and the intercept with the absorbance axis is calculated rather than fixed at zero concentration.
The equation for linear calculated intercept is:A=K1+K2*C
Linear Through Zero
If you expect the concentrations of your samples to be within the linear range of the calibration, select this option. This is often the case at low absorbance values and low concentration values. This calibration curve is forced to go through the point defined by the calibration blank which is set at zero absorbance and zero concentration. The equation used for zero intercept linear calibration is: C=K0(-K1*A)
In this expression, C is the concentration and A is the absorbance or emission, K1 is a proportionality coefficient, and K0 is the reslope coefficient, which is set to 1.0 for the initial calibration.
A calibration curve defined using this equation is forced to go through the point 0, 0, zero absorbance and zero concentration, defined by the blank. When two or more calibration standards are used, the method of least squares is used to determine K1 .
The blank is always analyzed first, and the reading obtained is automatically subtracted from the readings from all subsequent calibration standards.
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冰山
第29楼2013/05/03
应助达人哪位英语好的兄弟把这段给大家翻翻
yubo726(yubo726) 发表:Nonlinear Through Zero
If you expect the concentrations of your samples to be outside the linear range of the calibration curve , select this option. This option covers the widest concentration range. This calibration curve is forced to go through the point defined by the calibration blank which is set at zero absorbance and zero concentration. The equation for Nonlinear zero intercept is:
C=K0*(K1*A+K3*A*A)/(K2*A-1)
In this expression, C is the concentration, A is the observed absorbance or emission, K1, K2, and K3 are coefficients determined during the calibration procedure, and K0 is the reslope coefficient that is set equal to 1.0 during the initial calibration. When the number of calibration standards used exceeds the number of coefficients, the method of least squares is used to determine the coefficients.
The blank is always analyzed first, and the reading obtained is automatically subtracted from the readings from all subsequent calibration standards.
Linear, Calculated Intercept
If you expect the concentrations of your samples to be within the linear range of the calibration curve, select this option. This is often the case at low absorbance values and low concentration values. The calibration blank is treated as another calibration standard by the system and the intercept with the absorbance axis is calculated rather than fixed at zero concentration.
The equation for linear calculated intercept is:A=K1+K2*C
Linear Through Zero
If you expect the concentrations of your samples to be within the linear range of the calibration, select this option. This is often the case at low absorbance values and low concentration values. This calibration curve is forced to go through the point defined by the calibration blank which is set at zero absorbance and zero concentration. The equation used for zero intercept linear calibration is: C=K0(-K1*A)
In this expression, C is the concentration and A is the absorbance or emission, K1 is a proportionality coefficient, and K0 is the reslope coefficient, which is set to 1.0 for the initial calibration.
A calibration curve defined using this equation is forced to go through the point 0, 0, zero absorbance and zero concentration, defined by the blank. When two or more calibration standards are used, the method of least squares is used to determine K1 .
The blank is always analyzed first, and the reading obtained is automatically subtracted from the readings from all subsequent calibration standards.
