+关注 私聊

  • guocc007

    第11楼2009/12/11

    呵呵，李发美是位女老师，一位老太太

    windeast(windeast) 发表:一般是丙酮的，十年前额就是用这个标记淌度，叫做电渗流标记物。
    通常的研究都是用时间来进行比较和表征的，所以有个t=tm-t0的公式，这个是套用的色谱的。
    李发美这小子还越做越发迹了。不错 哈哈。

0
    +关注 私聊

  • userwzy

    第12楼2009/12/11

    Use acetone as a marker, and calculate the EOF according to the migration time, column length and voltage.
    If the EOF is very low, use 3 injection method. First, injection No.1 then flash with low pressure for several minutes, and then injection No. 2 and flash with some pressure and time, and add a volatege migrate several minutes, at last injection No. 3, then flush the column until the 3 peaks were shown. At last, according the distance difference between peak 1 and 2 and peak 2 and 3, migrate time and voltage calculte the EOF. it's an old paper, I think you can find it.

0
  • 分
  • 该帖子已被版主-sunpengwjh加5积分，加2经验;加分理由:积极参与讨论！
    +关注 私聊

  • guocc007

    第13楼2009/12/12

    谢谢这位热心的版友，非常感谢！

    userwzy(userwzy) 发表:Use acetone as a marker, and calculate the EOF according to the migration time, column length and voltage.
    If the EOF is very low, use 3 injection method. First, injection No.1 then flash with low pressure for several minutes, and then injection No. 2 and flash with some pressure and time, and add a volatege migrate several minutes, at last injection No. 3, then flush the column until the 3 peaks were shown. At last, according the distance difference between peak 1 and 2 and peak 2 and 3, migrate time and voltage calculte the EOF. it''s an old paper, I think you can find it.

0
    +关注 私聊

  • windeast

    第14楼2009/12/12

    哈哈 我才知道呢，见笑了，我毕业的时候搜了几篇她做手性拆分的。不过那时电泳做手性拆分已经沦落了，做得好也只能发分析化学的研究简报了。

    guocc007(guocc007) 发表:呵呵，李发美是位女老师，一位老太太

0
  • 分
  • 该帖子已被版主-sunpengwjh加2积分，加2经验;加分理由:积极参与讨论！