应助达人

Selective Automated Solid Phase Extraction of Patulin from Apple products Using Molecularly Imprinted Polymers

应助达人

VALIDATION OF ANALYTICAL METHODS TO DETERMINE THE CONTENT OF AFLATOXIN, OCHRATOXIN AND PATULIN IN FOODSTUFFS OF VEGETABLE ORIGIN

应助达人

Solid Phase Extraction (SPE) of Patulin

应助达人

Analysis of Patulin in Pear- and Apple-Based Foodstuffs by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry

A liquid chromatography electrospray ionization tandem mass spectrometry method for the determination of patulin in apple- and pear-based foodstuffs was developed. The sample preparation is based on the QuEChERS procedure involving an initial extraction step with water and acetonitrile, followed by a partitioning step after the addition of magnesium sulfate and sodium chloride. The cleanup was performed by using dispersive solid-phase extraction with a mixture of magnesium sulfate, primary secondary amine sorbent, and n-octadecylsiloxane sorbent added together to the extract. The cleaned extract was finally evaporated and reconstituted in water prior to injection. Quantitation was performed by isotope dilution using ((13)C(7))-patulin as internal standard. The method was first fully validated in three different baby food products including apple-pear juice, apple-pear puree, and infant cereals. Then the scope of application of the method was extended to pear concentrate, raw apples, apple flakes (naturally contaminated), dried apples, and yogurt. The sensitivity achieved by the method in all matrices gave limits of detection (LOD) and quantitation (LOQ) of �0.5 and �10 μg/kg, respectively, which was compliant with maximum levels settled in Commission Regulation (EC) No. 1881/2006. Method performances for all matrices also fulfilled the criteria established in the CEN/TR 16059:2010 document. Indeed, recoveries were within the 94-104% range; relative standard deviations of repeatability (RSD(r)) and intermediate reproducibility (RSD(IR)) were �7.5 and �13.0%, respectively, and trueness in an infant apple drink (FAPAS 1642) was measured at 99%.

看样用QuEChERS方法可以进行前处理。

应助达人

Patulin in juice and solid samples by HPLC

腐坏水果不都用来做果汁吗？

应助达人

做果汁的水果的只是品质差一点，比如小一点，大小不一等，不是坏果子。

说是发酵需要，不知有木有道理

应助达人

果汁是不用发酵的。但如果做水果醋或酒的话，需要发酵，但也有选择未坏的果子。

木有才(xgy2005) 发表:说是发酵需要，不知有木有道理

现在看来越来越多的人关注健康了，所以各种检测也是随之产生了的，黄曲霉毒素听过，棒曲霉还是第一次听到检测啊。