我是学食品专业的，研究方法，试验进展，仪器信息等方面的消息我都需要。研究方法和试验进展一般是在中英文文献中找，我们这里中文的主要有维普、万方和CNKI，英文的我主要用Science direct和Blackwell Synergy,其他的数据库我们也有，不过由于专业的原因，我主要用这两个，我们图书馆这两个数据库的资源也比较全面。仪器信息主要是从百度上搜。

最好有电子版的

目前我是纳米材料专业的
使用数据库主要是中国期刊网、WOS、ACS、elsvier、Wiley等，但是Nature系列查找不便
我需要下面这篇文章，谢谢（我的邮箱：jingtianwoshishei@x263.net）
W. D. Volkmuth and R. H. Austin, Nature ~London! 358, 600 ~1992.

一般基础性的、完全不懂的东西就上期刊网找点辅助信息，看书通常很费事
课题方向的把握主要是通过SCI检索，然后有兴趣的再寻找全文
实验技术找专业网站和仪器信息网等

Nature 358, 600 - 602 (13 August 1992); doi:10.1038/358600a0

DNA electrophoresis in microlithographic arrays
W. D. Volkmuth & R. H. Austin
Department of Physics, Princeton University, Princeton, New Jersey 08544, USA

WE have used optical microlithography to fabricate capped quasi-two-dimensional obstacle courses in SiO2. We report here observations using epifluorescence microscopy of the electrophoresis and length fractionation of large DNA molecules confined in arrays. Simple reptation theory, based on the work of deGennes1, predicts that at low electric fields the electrophoretic mobility of a polymer of length L much greater than the persistence length p scales inversely with L (ref. 2). But elongation of the coil in the matrix at sufficiently strong electric fields3 results in a length-independent electrophoretic mobility4,5. The application of suitably timed pulsed electric fields restores the fractionating power of gels for long molecules6 but the protocols of pulsed-field electrophoresis are semi-empirical because the complex and ill-understood gel matrix plays a critical role in fractionation. Microlithographically constructed obstacle arrays, with their low dimensionality, small volume and extremely reproducible topography, will make it possible to understand the motion and fractionation of large polymer molecules in complex but well characterized topologies.

References
1. DeGennes, P. Scaling Concepts in Polymer Physics 4th edn, 223−233 (Cornell Univ. Press, Ithaca, New York, 1991).
2. Lerman, L. S. & Frisch, H. L. Biopolymers 21, 995−997 (1982). | PubMed | ISI | ChemPort |
3. Smith, S. B., Aldridge, P. K. & Callis, J. B. Science 243, 203−206 (1989). | PubMed | ISI | ChemPort |
4. Hervet, H. & Bean, C. P. Biopolymers 26, 727−742 (1987). | PubMed | ChemPort |
5. Lumkin, O. J., Dejardin, P. & Zimm, B. H. Biopolymers 24, 1573−1593 (1985). | PubMed | ISI | ChemPort |
6. Schwartz, D. C. & Cantor, C. R. Cell 37, 67−75 (1984). | Article | PubMed | ISI | ChemPort |
7. Hagerman, P. J. A. Rev. Biophys. biophys. Chem. 17, 265−286 (1988). | ChemPort |
8. Morikawa, K. & Yanagida, M. J. Biochem. 89, 693−696 (1981). | PubMed | ISI | ChemPort |
9. Bustamante, C. A. Rev. Biophys. biophys. Chem. 20, 415−446 (1991). | ChemPort |
10. Schwartz, D. C. & Koval, M. Nature 338, 520−522 (1989). | Article | PubMed | ISI | ChemPort |
11. Schurr, J. M. & Smith, S. B. Biopolymers 29, 1161−1165 (1990). | ChemPort |
12. Holmes, D. & Stellwagen, N. Electrophoresis 11, 5−15 (1990). | PubMed | ChemPort |
13. Deutsch, J. M. & Madden, T. L. J. chem. Phys. 90, 2476−2485 (1989). | ChemPort |
14. Manning, G. S. J. chem. Physics 51, 924−933 (1969). | ChemPort |
15. Kozak, M. W. & Davis, E. J. Langmuir 6, 1585−1590 (1990). | ChemPort |
16. Serwer, P. Electrophoresis 10, 327−331 (1989). | PubMed | ChemPort |
17. Lalande, M. et al. Nucleic Acids Res. 16, 5427−5437 (1988). | PubMed | ChemPort |
18. Song, L. & Maestre, M. F. J. biomolec. Struc. Sterodynamics 9, 87−99 (1991). | ChemPort |
19. Wallis, G. & Pomerantz, D. I. J. appl. Phys. 40, 3946−3949 (1969). | ChemPort |

呵呵，你倒是一点也不客气，上来就要文章啊！
是这个文章吧？
你自己查收一下邮件吧。