Try initializing the Agilent ChemStation spooler by typing the following command on the Agilent ChemStation command line:
_LoadServiceResetPrinting
The Agilent ChemStation spooler prompts you whether to cancel all pending print jobs. If you do not want to cancel the pending jobs, press No. If the printing error comes again, you need to save your work, close all applications, and restart Windows and the Agilent ChemStation to reinitialize the operating environment.

找到了一个方法 在online时设置不要连接打印机

这样先解决问题，很好的。