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  • sabrina2007

    第11楼2007/04/27

    我也有这问题,谢谢!

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  • 剑神一笑

    第12楼2007/04/29

    出线了很正常,只要不影响实验数据就行

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  • tianjiu

    第13楼2007/05/01

    这个问题我还没有遇到过!你解决了也和大家说一说!

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  • chemtech33

    第14楼2007/05/01

    The editor said that the problem could be solved by changing the column. So the problem is not relate to the mobile phase.

    Otherwise, which detector is used in your LC??

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  • guansong

    第15楼2011/08/11

    (chemtech33) 发表:The editor said that the problem could be solved by changing the column. So the problem is not relate to the mobile phase.

    Otherwise, which detector is used in your LC??

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  • guansong

    第16楼2011/08/11

    你懂不?换柱子,与流动相无关,你是大哥!

    (chemtech33) 发表:The editor said that the problem could be solved by changing the column. So the problem is not relate to the mobile phase.

    Otherwise, which detector is used in your LC??

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  • 蓝人

    第17楼2011/08/12

    应助达人


    赞同这个观点

    (hengjiu) 发表:请说明你的流动相及出峰时间.若在2分钟内一般是溶剂峰.明白原理后自己去想吧,流动相一般认为做为参照的基准,流动相也有紫外吸收.若你的样品中有不具有紫外吸收的流动相或紫外吸收强度比流动相紫外吸收低就后出倒峰了.比如你拿甲醇水溶液(甲醇:水=80:20)做流动相,而你样品是水溶解的,这时就会出现倒峰.

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