磷酸化真核翻译延长因子2抗体说明书

中文名称 磷酸化真核翻译延长因子2抗体 英文名称 Phospho-EEF2 (Thr57) 别 名 EEF2 (phospho T57); p-EEF2 (phospho T57); EEF2; EF2; Elongation factor 2; Eukaryotic translation elongation factor 2; Eukaryotic Elongation Factor-2; EF2_HUMAN; EF-2. 供 应 商 远慕生物 产品类型 磷酸化抗体 研究领域 肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子 表观遗传学 抗体来源 Rabbit 克隆类型 Polyclonal 交叉反应 Human, Mouse, 产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 磷酸化真核翻译延长因子2抗体（石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分 子 量 95kDa 细胞定位 细胞浆 性 状 Lyophilized or Liquid 浓 度 1mg/1ml 免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human EEF2 around the phosphorylation site of Thr56 亚 型 IgG 纯化方法 affinity purified by Protein A 储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 磷酸化真核翻译延长因子2抗体产品介绍 background: EEF2 is a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. Function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Subcellular Location: Cytoplasm. Post-translational modifications: Phosphorylation by EF-2 kinase completely inactivates EF-2. Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis. ISGylated. Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily. Database links: UniProtKB/Swiss-Prot: P13639.4 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.