磷酸酶/磷酸二酯酶家族成员6抗体使用说明书

中文名称 磷酸酶/磷酸二酯酶家族成员6抗体 英文名称 ENPP6 别 名 B830047L21Rik; E-NPP 6; Ectonucleotide pyrophosphatase/phosphodiesterase 6; ectonucleotide pyrophosphatase/phosphodiesterase family member 6; ENPP 6; NPP 6; NPP6; ENPP6_HUMAN. 供 应 商 远慕生物 研究领域 肿瘤 细胞生物 神经生物学 激酶和磷酸酶 新陈代谢 抗体来源 Rabbit 克隆类型 Polyclonal 交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, 产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 磷酸酶/磷酸二酯酶家族成员6抗体（石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分 子 量 46kDa 细胞定位 细胞膜 分泌型蛋白 性 状 Lyophilized or Liquid 浓 度 1mg/1ml 免 疫 原 KLH conjugated synthetic peptide derived from human ENPP6 亚 型 IgG 纯化方法 affinity purified by Protein A 储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 磷酸酶/磷酸二酯酶家族成员6抗体产品介绍 background: NPP6 is a 440 amino acid member of the nucleotide pyrophosphatase/phosphodiesterase family. NPP6 is a secreted and single-pass type I membrane protein. Predominantly expressed in brain and kidney, NPP6 is a choline-specific glycerophosphodiester phosphodiesterase. NPP6 can hydrolyze the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine, glycerophosphorylcholine, sphingosylphosphorylcholine and lysophosphatidylcholine (LPC). NPP6 has been found to have a preference for LPC with polyunsaturated or short fatty acids. The gene encoding NPP6 maps to human chromosome 4, which consists of approximately 6% of the human genome and nearly 900 genes. Chromosome 4 is associated with Huntington's disease, FGFR-3, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. Function: ENPP6 is a choline-specific glycerophosphodiester phosphodiesterase, which hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5 prime-monophosphate. It hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. ENPP6 has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. ENPP6 also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. ENPP6 is predominantly expressed in kidney and brain. Subunit: Homodimer; disulfide-linked. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Secreted. Note: A minor secreted form also exists. Tissue Specificity: Predominantly expressed in kidney and brain. In the kidney, expressed specifically in the proximal tubules and thin descending limbs of Henle (at protein level). Similarity: Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. Database links: UniProtKB/Swiss-Prot: Q6UWR7.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.