磷酸化EphrinB抗体实验步骤

中文名称 磷酸化Ephrin B抗体 英文名称 phospho-Ephrin B (Tyr329) 别 名 Ephrin B (phospho Y329); p-Ephrin B (phospho Y329); phospho-Ephrin B1 (Tyr329); phospho-Ephrin B2(Tyr316); CEK5 ligand; EFNB1; ELK L; EphrinB; Ephrin-B; ELK ligand; ELKL; EPH related receptor tyrosine kinase ligand 2; Ephrin B Precursor; Ephrin B1; Ephrin B2; LERK 2; LERK2; EFNB1_HUMAN; EFNB2_HUMAN. 供 应 商 远慕生物 产品类型 磷酸化抗体 研究领域 肿瘤 细胞生物 神经生物学 生长因子和激素 抗体来源 Rabbit 克隆类型 Polyclonal 交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, 产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 磷酸化Ephrin B抗体（石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分 子 量 34kDa 细胞定位 细胞膜 性 状 Lyophilized or Liquid 浓 度 1mg/1ml 免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Ephrin B around the phosphorylation site of Tyr329 亚 型 IgG 纯化方法 affinity purified by Protein A 储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 磷酸化Ephrin B抗体产品介绍 background: Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase. Subunit: Interacts with GRIP1 and GRIP2. Binds to Nipah virus G protein. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments. Similarity: Belongs to the ephrin family. Contains 1 ephrin RBD (ephrin receptor-binding) domain. Database links: Entrez Gene: 1947 Human Entrez Gene: 1948 Human Entrez Gene: 13641 Mouse Entrez Gene: 13642 Mouse Entrez Gene: 25186 Rat Omim: 300035 Human Omim: 600527 Human SwissProt: P52799 Human SwissProt: P98172 Human SwissProt: P52795 Mouse SwissProt: P52800 Mouse SwissProt: P52796 Rat SwissProt: Q6P7B6 Rat Unigene: 144700 Human Unigene: 149239 Human Unigene: 44398 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.