转录因子ERF抗体应用说明

中文名称 转录因子ERF抗体 英文名称 ERF 别 名 ERF; ERF_HUMAN; ETS domain containing transcription factor ERF; ETS domain-containing transcription factor ERF; Ets2 repressor factor; PE 2; PE-2; PE2. 供 应 商 远慕生物 研究领域 细胞生物 转录调节因子 表观遗传学 抗体来源 Rabbit 克隆类型 Polyclonal 交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, 产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分 子 量 59kDa 细胞定位 细胞核 性 状 Lyophilized or Liquid 浓 度 1mg/ml 免 疫 原 KLH conjugated synthetic peptide derived from human ERF/Ets2 Repressor Factor:65-160/548 亚 型 IgG 纯化方法 affinity purified by Protein A 储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. PubMed PubMed 产品介绍 ABT1 (activator of basal transcription 1) is a nuclear protein that associates with the TATA-binding protein (TBP) and enhances basal transcription activity of class II promoters. ABT1 associates with TBP in HeLa nuclear extracts in vitro. Another protein, designated ERF, is a member of the Ets family of transcription factors. The members of the Ets family are grouped because they share a highly conserved DNA binding domain. These factors are involved in growth factor pathways and regulate both proliferation and differentiation. ERF (Ets-2 repressor factor) is a ubiquitously expressed Ets-domain protein that exhibits strong transcriptional repressor activity, suppresses Ets-induced transformation and is regulated by MAPK phosphorylation. ERF transcription may be regulated by Ets-domain proteins. Additionally, modulation of ERF activity is involved in the transcriptional regulation of genes activated during entry into G1 phase.