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Monoclonal antibody (mAb) arrays ware made using purified proteins andascites fluid. The mAbs were immobilized with the antigen recognition sites oriented away from the chip surface, usingbiotinylated goat-anti-mouse antibody (Fc) to capture the mAbs. Equlibrium binding o antigen to the mAbs was then measured for a range of anigen concentrations.
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