高效液相色谱法同时检测食品中十种食品添加剂

摘 要：本方法建立一种高效液相色谱法快速分离检测食品中十种食品添加剂的方法，采用C18，5μ，4.6 × 150mm 色谱柱；0.02mol/L 乙酸铵(pH6.0)- 甲醇为流动相，梯度淋洗；柱温30℃；流速1.0ml/min；检测波长230nm 和 258nm。十种添加剂在0.0025～0.050mg/ml 内线性相关系数r ＞ 0.9999，回收率为86.4%～100.4%，相对标准偏 差RSD 为0.1%～0.9%，检测限为0.2～0.8mg/kg。 关键词：高效液相色谱法；食品；食品添加剂 Simultaneous Determination of Ten Food Additives by High Performance Liquid Chromatography LIN Qin，HUANG Hong-xia (Fujian Provincial Central Inspection Institute，Fuzhou 350002, China) Abstract：A rapid method for simultaneous determination of ten food additives in food by high performance liquid chromatography was developed. Liquid chromatographic separation was performed on an C18, 5μ, 4.6×150mm column. The mobile phase is 0.02mol/L ammoninum acetate (pH6.0)-methanol(gradient elution) with column temperature 30℃ flow rate 1.0ml/min, and detection wavelengths 230nm and 258nm. The linear range for ten food additives is 0.0025～ 0.050mg/ml; r>0.9999, while recoveries of ten food additives are 86.4%～100.4%, RSD 0.1%～0.9%(n=4), and detection limits0.2～0.8mg/kg. Key words：HPLC；food；food additives