TSK-GEL SWXL系列色谱柱(尺寸排阻色谱法)对高分子量标准蛋白混合样品的分析

色谱柱 : TSK-GEL SWXL series 流动相 : 20mmol/L Phosphate buffer + 0.3mol/L NaCl (pH7.0) 流速 : 0.5mL/min 检测 : UV (280nm) 温度 : 25C 进样体积 : 10μL 样品 : standard protein (high molecular weight) 液相色谱仪 : Agilent 1120 Compact LC (gradient auto system) 样品组成 : 1. Mouse monoclonal antibody, IgM (M.W.:900KDa, in house, 3g/L) 2. Thyroglobulin (M.W.:667KDa, bovine thyroid, Sigma T1001, 3g/L) 3. Glutamate dehydrogenase (M.W.:280KDa, Wako pure chemicals, Japan, 306-50844, 4g/L) 4. Mouse monoclonal antibody, IgG1 (M.W.:150KDa, in house, 2g/L) 5. Conalbumin (M.W.:47KDa, Wako pure chemicals, Japan, 016-17073, 4g/L)