用户论文(2005)：rhBMP_2纳米缓释系统的制备及其对骨髓间充质干细胞增殖和分化的影响

【中文摘要】 目的:制备甲基丙烯酸缩水甘油酯右旋糖酐(dex-GMA)载重组人骨形态发生蛋白2(rhBMP2)凝胶纳米微球(NPs)缓释系统,检测其理化及生物学特性。方法:采用改良乳化溶液聚合法,制备rhBMP2纳米微球(rhBMP2-dex-NPs);观察其形态、粒径与稳定性,检测其载药、释药特征;采用体外细胞培养技术,将rhBMP2-dex-NPs和兔骨髓间充质干细胞一起培养,MTT法检测其对细胞增殖状况的影响,碱性磷酸酶(ALP)检测试剂盒检测细胞ALP活性,以反应rhBMP2-dex-NPs对其分化状况的影响,并与单纯rhBMP2的作用进行比较;实验按照培养液中所含成分不同分为4组:培养液中加入单纯rhBMP2(A组)、加入rhBMP2-dex-NPs(B组)、加入空白NPs(C组)和对照组(D组),其中A、B2组均分别按照rhBMP2的不同量设置100μg/L、200μg/L、400μg/L3个浓度组,采用SPSS10.0对结果进行统计学分析。结果:rhBMP2-dex-NPs大小均匀,粒径分析表明,80%的纳米球粒径在20nm左右,包封率高达83%,80%的rhBMP2在前12d释放;其具有良好的生物学活性,能... 【英文摘要】 PURPOSE: To prepare and study the recombinant human bone morphogenetic protein-2 loaded dextran-based hydrogel nanospheres (rhBMP2-dex-NPs) sustained release system, and to evaluate its biological effects on cultured rabbit bone mesenchymal stem cells(BMSCs). METHODS: The rhBMP2-dex-NPs were prepared by improved emulsion polymerization method. Their morphology, size and size distribution, encapsulated ratio and stability were assessed by routine procedure. Dynamic dialysis method was used to determine the r...