专家著述(2005)：电场驱动微悬臂生物传感器及对DNA的快速、高灵敏度检测

【中文摘要】 微悬臂列阵传感器在生物检测方面具有快速、痕量和非标记的特性. 我们以镀金并在其上固定了DNA探针的微悬臂为正极,在靶杂交液槽内引入另一电极作为负极,构成电场驱动微悬臂DNA生物传感器. 对该传感器系统施加静电场,驱动DNA分子朝正极迁移,使溶液中的DNA分子富集在微悬臂上,促进DNA分子的杂交. 结果表明:a. DNA在微悬臂上的杂交时间仅需3 min,加快了微悬臂生物传感器对DNA分子的检测速度;b. 提高了微悬臂生物传感器的灵敏度,可以检测到皮克级的DNA分子. 【英文摘要】 Microcantilever array biosensor shows peculiarities of high speed, trace detection and label-free in bioanalysis. A method to increase the detection sensitivity and to speed up analysis of microcantilever array biosensor is reported. The gold covered microcantilever array immobilized by DNA probes is applied as a microcantilever array biosensor with electrostatic field. Microcantilever arry is acted as anode in hybridization solution cell where DNA molecules are removed to and congregated around anode, so t...