专家著述(2004)：单分子PCR产物错误率分析

【中文摘要】 碱基错配致使PCR扩增产物中存在突变序列 .大量模板PCR扩增时突变序列所占的比例较低 ,对随后进行的PCR产物分析影响不大 ,但当对微量甚至单个模板DNA扩增时 ,情况则完全不同 .对单分子PCR产物的错误率进行了理论分析 ,结果表明 :根据实验目的和条件 ,选择忠实性不同的聚合酶是十分关键的 【英文摘要】 Mismatches during amplification result in some mutations in the PCR products. The simulation indicates that the rate of mutation is quite low and has no influence on the consequent sequencing analysis when template DNA molecules are abundant, but it is not the case when the PCR amplification is performed with single or few molecules as templates. The error rates of single molecule PCR under different conditions showed that it is essential to select different polymerases according to different experimental p...