专家著述(2001)：基于AFM成像的DNA长度的计算机统计处理

【中文摘要】 利用计算机图像处理技术 ,对自然沉积DNA和拉直DNA分子AFM图像的长度进行了自动统计测量 ,并与DNA分子的理论长度进行了比较。该方法在DNA分子的AFM图像的基础上 ,将DNA分子拟合成曲线 ,用插值法求得DNA分子长度 ,整个过程实现了自动化。结果表明 ,该方法测得自然沉积的某DNA分子长度为 (180 .4± 16.4 )nm ,与理论长度 185 .0nm相当符合。该方法与拉直法和直接法求DNA分子长度相比是一次改进 ,也为其它基于AFM图像的线状物体求长度提供了一种新的测量方法。对另一组拉直DNA测量的结果表明 :拉直后DNA分子长度为 (34 3.6± 2 0 .7)nm ,与理论长度 30 7.0nm相比明显变长 ,这表明对自然沉积DNA进行长度测量的必要性 ,保证了DNA分子的长度不受拉直后有所伸长的影响。 【英文摘要】 Taking advantage of image processing technology, the contour length of DNA molecule was measured automatically by a computer. Based on the AFM image of DNA, the topography of DNA was simulated into a curve. Then the DNA length was measured automatically by inserting mode. It was shown that the experimental length of a naturally deposited DNA (180.4±16.4nm) was well consistent with the theoretical length (185.0nm). Comparing to other methods, the present approach had advantages of precision and automatism. T...