专家著述(1997)：高温DNA聚合酶I的Klenow片段在乙醇中变性的AFM研究

【中文摘要】 本文利用原子力显微镜（ＡＦＭ），对高温ＤＮＡ聚合酶Ｉ和普通Ｅ．ｃｏｌｉ的ＤＮＡ聚合酶Ｉ的Ｋｌｅｎｏｗ片段在乙醇中变性的对比研究，发现在两种ＤＮＡ聚合酶中都存在结构较紧密的“核”。通过对吸附在云母上的两种酶进行１—１０个小时的处理发现高温聚合酶大多由３块“核”组成，而普通的ＤＮＡ聚合酶由２块“核”组成。这些“核”可能与蛋白的折叠过程中存在的相对稳定的结构区域有关，这对直观了解蛋白折叠过程有一定意义。 【英文摘要】 Atomic Force Microscopy(AFM) has been used to study the changes of main structure in the denaturating process of Bacillus stearothermophilus T 3468 DNA polymerase I Klenow Fragment( Bet pol I KF) and its homologous protein( Escherichia coli DNA polymerase Klenow Fragment) in alcohol.For comparison,images of Bst pol I KF have also been obtained by tapping mode AFM in air,and its contour looks like a kidney or a pear,which is similar to that of Escherichia coli DNA polymerase I Klenow Fragment obtain...