用户论文(2005)：葛仙米藻红蛋白的分离、纯化与结构及生物活性研究

【中文摘要】 葛仙米以其资源稀少、营养价值丰富,独特的生物活性和药理作用而引起人们广泛地关注。葛仙米作为一种食药同源的传统食品已有几千年的历史,但对其主要活性成分的研究很少。近年来,仅少数人对其生理和基本的生化组成进行了研究。为探讨葛仙米中的生物活性成分,本文以野生葛仙米藻红蛋白为研究对象,在系统研究葛仙米藻红蛋白的提取、分离和纯化的基础上,利用生物大分子研究的最新技术和现代分离、分析手段,尝试揭示葛仙米藻红蛋白的结构与生物活性及二者间的关系。其主要结果如下: 1 葛仙米的生态条件及分布规律与生化组成研究 以湖北省鹤峰县走马镇野生葛仙米为研究对象,对其生态条件及分布规律与生化组成作了较系统的分析。测定了其水分、蛋白质、脂肪、多糖、类胡萝卜素、叶绿素、矿物元素的含量。 2 葛仙米藻红蛋白的提取、分离、纯化和鉴定 以脱脂葛仙米为原料,采用盐溶和盐析的方法,首次从葛仙米中制备出清蛋白、球蛋白和盐溶蛋白,并确定了适合葛仙米藻胆蛋白提取制备的最佳工艺,其最佳工艺条件为,缓冲液pH值为7.3、浸提时间4.3h、盐浓度0.21mol/L,葛仙米藻胆蛋白最大得率为7.13%。 利用DEAE... 【英文摘要】 The significant biological and pharmacological activity of Nostoc Sphaeroids kutz with abound nutrition and infrequent distribution had been paid people''s much attention. Although NSK as a kind of food and medicine function had been used for several thousand years, the main active component of activity and function is little investigated. In recent years, it was by a few people that its partial physiology and primary biochemistry composition was reported. In order to ascertain the active component of NSK