利用RP－HPLC分析、纯化蛋白质和多肽的手册

利用RP－HPLC分析、纯化蛋白质和多肽的手册Table of Contents Introduction . .2 Mechanism of Interaction . .4 The Role of the Column in Polypeptide Separations . .8 Analytical Conditions: The Role of the Mobile Phase and Temperature .17 Reversed-Phase HPLC/Mass Spectrometry . .26 The Role of Reversed-Phase HPLC in Proteomic Analysis . .30 Examples of the Use of Reversed-Phase HPLC in the Analysis of Polypeptides .32 HPLC as a Tool to Purify and Isolate Polypeptides . .40 Viral Inactivation During Reversed-Phase HPLC Purification . .50 Appendices Appendix A: Column Characteristics . .52 Appendix B: The Care and Maintenance of Reversed-Phase Columns . .53 Appendix C: The Effect of Surfactants on Reversed-Phase Separations . .56 Appendix D: Ion Exchange Chromatography: Orthogonal Analytical Techniques .. .58 Appendix E: The Effect of System Hardware on Reversed-Phase HPLC Polypeptide Separations . .60 Technical References . .62