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【中文摘要】 采用薄膜蒸发法制备盐酸小檗碱脂质体,紫外分光光度———透析法测定脂质体包封率;以盐酸小檗碱脂质体包封率为指标,考察影响脂质体包封率的各因素.结果表明,制备盐酸小檗碱脂质体的最佳条件为孵化温度60℃,孵化时间30 m in,胆固醇3.3 mg.mL-1,此时包封率达到最高,且脂质体的包封率随着卵磷脂浓度的增加呈线性增加. 【英文摘要】 Berberine hydrochloride liposomes were prepared by thin-film evaporation method.The encapsulation efficiency of liposome was determined by UV spectrophotometric method after liposome was dialyzed.The various influences on the encapsulation efficiency were studied.The experimental results demonstrated that the liposome could get highest encapsulation efficiency when the hatched temperature was at 60℃,the hatched time was 30min,the cholesterol concentration was 3.3mg·mL~(-1),and the encapsulation efficiency...
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