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Spectra-structure relationships were investigated for estimating the anomeric configuration, residues and type of linkages of linear and branched trisaccharides using 13C-NMR chemical shifts. For this study, 119 pyranosyl trisaccharides were used that are trimers of the or anomers of D-glucose, D-galactose, D-mannose, L-fucose or L-rhamnose residues bonded through or glycosidic linkages of types 1→2, 1→3, 1→4,or 1→6, as well as methoxylated and/or N-acetylated amino trisaccharides.
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