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Introduction: Marijuana, one of the most widely abused drugs, after alcohol, is derived from the Cannabis Sativa plant. There are more than 400 chemicals in the cannabis plant. The Δ9-tetrahydrocannabinol (THC) is the most psychoactive of the various forms of THC. Marijuana is most often used in cigarette form, the user inhaling the marijuana smoke. THC and other forms of cannabinoids are lipid soluble and can enter into body tissues rapidly. THC is rapidly metabolized to 11- hydroxy-Δ9-tetrahydocannabinol (11-OH-THC), which is then converted into 11-nor-Δ9-THC-9-carboxylic acid (THC-COOH). Detection of THC metabolites in urine, primarily THC-COOH, impairment. Testing for THC and its metabolites in blood can give a better indication of recent drug usage and can be of merit when testing for impairment. This application note shows an effective SPE method for the extraction of THC and key metabolites from human blood and the GC/MS analysis of these compounds.
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