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Abstract – Ewe’s milk fat obtained from the milk of five different herds were studied to characterize main and minor fatty acids using a combination of gas chromatography and mass spectrometry. In order to assess monounsatured trans fatty acids content, fatty acid methyl esters (FAME) were fractionated by silver ion thin-layer chromatography prior to gas chromatography analysis. Trans fatty acids (TFA), including conjugated linoleic acid, represented 4.9% of the total FAME; most of them belonged to C18:1 trans isomers (2.9%) with a small range of variation (2.5–3.2%). The distribution profile of trans-C18:1 molecules indicated that more than half were trans-11, whereas the proportion of potentially unhealthy compounds (trans-10 and trans-9 C18:1) was less than 10%. Mean trans-C16:1 content was 0.25% of total FAME. The sum of the different trans-C18:2 isomers (excluding conjugated linoleic acid) was 0.88% and C18:3 trans isomers represented 0.16%. 我们可以提供很齐全的脂肪酸FA,和脂肪酸甲酯FAME标准品 以及专用的脂肪酸分析柱CP-SIL 88毛细管柱
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