PerkinElmer 荧光光谱导论

PerkinElmer的用户手册——荧光光谱导论 An Introduction to Fluorescence Spectroscopy Table of Contents Table of Contents............................................................................................3 Luminescence and the nature of light.............................................................5 Fluorescence...................................................................................................7 Phosphorescence...........................................................................................11 Light scattering.............................................................................................14 Rayleigh-Tyndall scattering..................................................................14 Raman scattering...................................................................................14 Quantitation..................................................................................................16 Sensitivity.....................................................................................................18 Instrumental sensitivity.........................................................................18 Method sensitivity.................................................................................18 Absolute sensitivity...............................................................................18 Instrumentation.............................................................................................21 General..................................................................................................21 Light sources.........................................................................................22 Wavelength selection.............................................................................24 Detectors................................................................................................25 Read-out devices...................................................................................26 Sample holders......................................................................................26 Problems of high blank values......................................................................28 Cuvettes.................................................................................................28 Solvents and Reagents...........................................................................28 Other contaminants................................................................................29 Working with dilute solutions......................................................................30 General..................................................................................................30 Adsorption.............................................................................................30 Photo-decomposition.............................................................................31 Oxidation...............................................................................................31 Factors affecting quantitative accuracy........................................................32 Non-linearity..........................................................................................32 Temperature effects...............................................................................32 pH effects...............................................................................................33 Inner-filter effects..................................................................................33 Quenching..............................................................................................33 References....................................................................................................34