Rat Adenosine ELISA Kit

Rat Adenosine ELISA Kit FOR RESEARCH USE ONLY. Not for clinical diagnosis use CATALOG #:95797 INTRODUCTION ? For the quantitative determination of rat Adenosine concentrations. ? Detection of species: Rat ? Detection medium: serum, cell culture supernates. PRINCIPLE OF TEST The kit is for the quantitative level of Adenosine in the sample, adopt purified rat Adenosine antibody to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to Adenosine, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody - antigen - enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of Adenosine in the samples is then determined by comparing the O.D. of the samples to the standard curve.