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Rat VCAM-1 FOR RESEARCH USE ONLY CATALOG #:R6931 Assay range：25μg/L -800μg/L 96 determinations Purpose This kit allows for the determination of VCAM-1/CD106 concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat VCAM-1/CD106 level in the sample, use Purified Rat VCAM-1/CD106 antibody to coat microtiter plate wells, make solid-phase antibody, then add VCAM-1/CD106 to wells, Combined VCAM-1/CD106 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically
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