Rat tumor necrosis factor α（TNF-α）ELISA Kit

Rat tumor necrosis factor α（TNF-α）ELISA Kit FOR RESEARCH USE ONLY INTRODUCTION Assay range：10ng/L-360ng/L 96 determinations Purpose This kit allows for the determination of TNF- α concentrations in Rat serum, cell culture supernates and other biological fluids PRINCIPLE OF TEST The kit assay Rat TNF- α level in the sample ， use Purified Rat TNF- α antibody to coat microtiter plate wells, make solid-phase antibody, then add TNF- α to wells, Combined TNF- α antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TNF- α in the samples is then determined by comparing the O.D. of the samples to the standard curve.