锤子
Rat MURF1 FOR RESEARCH USE ONLY Assay range: 5 ng/L - 180 ng/L 96 determinations Purpose This kit allows for the determination of MURF1 concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat MURF1 level in the sample, use Purified Rat MURF1 antibody to coat microtiter plate wells, make solid-phase antibody, then add MURF1 to wells, Combined MURF1 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat MURF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
打开失败或需在电脑查看,请在电脑上的资料中心栏目,点击"我的下载"。建议使用手机自带浏览器。