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Rat IL-6 FOR RESEARCH USE ONLY Assay range:3pg/ml-120pg/ml 96 determinations Purpose This kit allows for the determination of IL-6 concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat IL-6 level in the sample,use Purified Rat IL-6 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-6 to wells, Combined IL-6 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue
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