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Rat IL-10 FOR RESEARCH USE ONLY Assay range:3 ng/L -100 ng/L 96 determinations Purpose This kit allows for the determination of IL-10 concentrations in Rat serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Rat IL-10 level in the sample, use Purified Rat IL-10 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-10 to wells, Combined IL-10 antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat IL-10 in the
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