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Rat secretory immunoglobulin A (sIgA) ELISA Kit Specimen requirements 1. serum- coagulation at room temperature 10-20 mins,centrifugation 20-minat the speed of 2000-3000 r.p.m. remove supernatant, If precipitationappeared, Centrifugal again. 2. plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. removesupernatant, If precipitation appeared, Centrifugal again. 3. Urine-collect sue a sterile container, centrifugation 20-min at the speed of2000-3000 r.p.m. remove supernatant, If precipitation appeared,Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluidReference to it.
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