运用高速逆流色谱制备分离和纯化吴茱萸的生物碱成分

Abstract：High-speed counter-current chromatography(HSCCC)with a two-phase solvent system composed of n-hexane–ethyl acetate–methanol–water system (5:5:7:5, v/v)was applied to the isolation and purification of alkaloids from the Chinese medicinal plant Evodia rutaecarpa (Juss.) Benth. Five kinds of alkaloids were obtained and yielded 28mg of evodiamine(I),19mg of rutaecarpine(II),21mg of evocarpine (III),16 mg of 1-methy-2-[(6Z,9Z)]-6,9-pentadecadienyl-4-(1H)-quinolone (IV),12mg of 1-methyl-2-dodecyl-4-(1H)-quinolone (V) from 180 mg of crude extract in a one-step separation, with the purity of 98.7%, 98.4%, 96.9%, 98.0%, 97.2%, respectively, as determined by high performance liquid chromatography (HPLC). The structures of these compounds were identified by HNMRand CNMR.