运用高速逆流色谱分离纯化紫花前胡香豆素成分

Abstract: A preparative high-speed counter-current chromatography (HSCCC) method for isolation and purification of coumarin compounds from the Chinese medicinal plant Peucedanum decursivum (Miq.) Maxim (Zihuaqianhu in Chinese) was successfully established by using light petroleum–ethyl acetate–methanol–water (5:5:7:4, v/v) as the two-phase solvent system. The upper phase of light petroleum–ethyl acetate–methanol–water (5:5:7:4, v/v) was used as the stationary phase of HSCCC. Nodakenetin (2.8 mg), 6.1 mg of Pd-C-IV, 7.3 mg of Pd-D-V, 4.7 mg of ostruthin, 7.8 mg of decursidin and 11.2 mg of decursitin C with the purity of 88.3%, 98.0%, 94.2%, 97.1%,97.8% and 98.4%, respectively, were separated successfully in one-step separation from 150 mg of crude sample from P. decursivum(Miq.) Maxim. After purified by HSCCC again with light petroleum–ethyl acetate–methanol–water (5:5:4:5, v/v) as the two-phase solvent system, the purity of (I) can reach 99.4%. The structures of all the compounds were identified by H-NMR and C-NMR.