运用高速逆流色谱法从太子参中分离纯化 Pseudostellarin B (cyclic peptide)

Abstract：Pseudostellarin B (cyclic peptide) was isolated and purified from the herbs of Pseudostellaria heterophylla (Miq.) Pax for the first time by high-speed counter-current chromatography (HSCCC) using a two-phase solvent system consisting of n-butanol–ethyl acetate–water (0.6:4.4:5,v/v).The technique can isolate mg levels of the target compound per run with purity better than 96%. The chemical structure of the compound has been positively confirmed by electrospray ionization time of flight (TOF) MS,H-NMR,C-NMR and H-C COSY analyses.