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简介：
Generic Name：Human Renin ELISA Kit. Purpose This kit allows for the determination of Renin concentrations in Human serum, blood plasma, and other biological fluids. Principle of the assay The kit assay Human Renin level in the sample，use Purified Human Renin antibody to coat microtiter plate wells, make solid-phase antibody, then add Renin to wells, Combined Renin antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at awavelength of 450 nm. The concentration of Renin in the samples is thendetermined by comparing the O.D. of the samples to the standard curve.
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