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Manual step: 1. Take out the reagents from freezer. Thaw them gently and place the tubes in the slots of cooling block on the VERSA100 deck. Automation steps: 2. Aspirate the required volume of each of the reagent number 1 to 4 (as shown in the table below) and dispense into the empty Master Mix (MM1) tube on the cooling block. Change tip for every reagent. 3. Mix the contents by pipetting twice with the tip in use. ... ...
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