新型氨基酸分析仪的研制及其初步应用

A200型氨基酸分析仪的分析原理建立在可靠的阳离子柱色谱分离和茚三酮柱后衍生检测的基础之上。不同于传统的氨基酸分析仪之处，首先在于A200设计了一种新液路，省去了惰气装置，实现了整机的小型与集成化。将包含控制软件与数据处理软件的工作站集成于机体一身，以大屏幕触摸式键盘进行操作，无需再配置台式计算机。研究成功一种分析体系，可连续自动测定水解与生理体液氨基酸而无需更换淋洗液和分离柱。与同类仪器相比试剂消耗降低50%以上;仪器的重量和体积减小至三分之一左右。仪器制造和运行成本显著降低。仪器的分析精度提高：峰保留时间RSD优于0.5%, 峰面积RSD优于1%。已成功应用于饲料，饮料，食品和临床分析包括蛋白质水解氨基酸和生理体液氨基酸的连续测定。对照分析结果表明该仪器操作简便，结果可靠。适合质量控制分析，也适合科学研究之用。 此外A200还为氨基酸分析仪－离子色谱仪技术的联用提供了现实可能性，将另文介绍。 A new generation of the amino acid analyzer A200 was realized in 2002. Based on the cation chromatographic separation and photometric detection at 570 and 440 nm after postcolumn derivatization with ninhydrin, the A200 is distinguished from the traditional amino acids analyzer by means of (1) the development of a new fluid-system without inert-gas-equipment; (2) the miniaturization of components to reduce reagent consumption up to 50%, to reduce volume and weight up to 60% ; (3) the development of a new workstation integrated on the A200 with controlling unit and chromatographic software; (4) the development of a novel separation method with one lithium-buffer system and one column for both hydrolyzed and physiological amino acids analyzer. The precision of the A200 for the retention time is RSD <0.5% and peak area RSD <1%. Applications for a lot of AOAC standards and real samples such as feedstuff, fish powder, maize powder, cobweb, win, beer, milk products and clinical samples etc. show that the A200 is suitable both for the routine and also for the research analysation of amino acids.