人11去氢血栓烷B2(11-DH-TXB2)ELISA试剂盒

Human 11-DH-TXB2 FOR RESEARCH USE ONLY Assay range：20pg/ml-480pg/ml 96 determinations Purpose This kit allows for the determination of 11-DH-TXB2 concentrations in Human serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Human 11-DH-TXB2 level in the sample, use Purified Human 11-DH-TXB2 antibody to coat microtiter plate wells, make solid-phase antibody, then add 11-DH-TXB2 to wells, Combined 11-DH-TXB2 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human 11-DH-TXB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.