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Human Epinephrine (EPI) Assay range:8 ng/L -200 ng/L 96 determinations Purpose This kit allows for the determination of EPI concentrations in Human serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Human EPI level in the sample, use Purified Human EPI antibody to coat microtiter plate wells, make solid-phase antibody, then add EPI to wells, Combined EPI antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human EPI in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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