人生长分化因子-11（GDF-11）ELISA试剂盒

Human GDF-11 FOR RESEARCH USE ONLY Assay range：5ng/L - 180ng/L 96 determinations Purpose This kit allows for the determination of GDF-11 concentrations in Human serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Human GDF-11 level in the sample, use Purified Human GDF-11 antibody to coat microtiter plate wells, make solid-phase antibody, then add GDF-11 to wells, Combined GDF-11 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human GDF-11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.