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Human FETU-A FOR RESEARCH USE ONLY Assay range:100ng/ml - 3200ng/ml 96 determinations Purpose This kit allows for the determination of FETU-A concentrations in Human serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Human FETU-A level in the sample,use Purified Human FETU-A antibody to coat microtiter plate wells, make solid-phase antibody, then add FETU-A to wells, Combined FETU-A antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human FETU-A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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