人锌转运体8抗体（ZNT8 Ab）ELISA试剂盒

Human ZNT8 Ab FOR RESEARCH USE ONLY Assay range：6.0ng/L – 160 ng/L 96 determinations Purpose This kit allows for the determination of ZNT8 Ab concentrations in Human serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Human ZNT8 Ab IgM level in the sample, use Purified antigen to coat microtiter plate wells, make solid-phase antigen, then add ZNT8 Ab to wells, Combined antigen which With HRP labeled, become antigen – antibody - enzyme-antigen complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human ZNT8 Ab in the samples is then determined by comparing the O.D. of the samples to the standard curve.