小鼠c-myc癌基因产物（c-myc）ELISA试剂盒

Mouse c-myc FOR RESEARCH USE ONLY Assay range：60pg/ml-1600pg/ml 96 determinations Purpose This kit allows for the determination of c-myc concentrations in Mouse serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Mouse c-myc level in the sample, use Purified Mouse c-myc antibody to coat microtiter plate wells, make solid-phase antibody, then add c-myc to wells, Combined c-myc antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Mouse c-myc in the samples is then determined by comparing the O.D. of the samples to the standard curve.