小鼠干扰素调节因子1（IRF1）

Mouse Interferon regulatory factor 1 (IRF1) FOR RESEARCH USE ONLY Assay range：10 ng/L -320ng/L 96 determinations Purpose This kit allows for the determination of IRF1 concentrations in Mouse serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Mouse IRF1 level in the sample, use Purified Mouse IRF1 antibody to coat microtiter plate wells, make solid-phase antibody, then add IRF1 to wells, Combined IRF1 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Mouse IRF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.